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Immunofixation electrophoresis is a technique that anchors (eg "fixes") a protein in situ after electrophoresis:
1. Proteins are separated by agarose gel electrophoresis.
2. Antiserum is overlaid directly onto the gel surface; it diffuses into the gel and interacts with antigen.
3. The gel is washed and unprecipitated, soluble proteins are removed. (The antigen-antibody complex is trapped within the gel matrix.)
4. The precipitated protein complexes are visualized with a protein stain.
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