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Unit Code 300009:
Zinc Protoporphyrin, Blood

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Method Description

Zinc protoporphyrin is measured on the Helena ProtoFluor-Z

hematofluorometer using a multi-channel front surface photofluorometer.

When the sample is exposed to 415 nm light, zinc protoporphyrin (ZPP)

is excited and emits light at 595 nm. A second lens/filter system collects,

filters and focuses the 595 nm light onto a photomultiplier tube (PMT).

The PMT produces a current level in response to the light reaching it,

which is proportional to the ZPP/heme ratio. During the 5 second reading,

over 1,000 light-level readings are taken and averaged by the micro-

computer and a value is displayed in umol ZPP/mol heme. (Operator's

Manual:  Helena ProtoFluor-Z 7/2006)

Performing Laboratory Location

Rochester

Key