For cytology analysis, brushing specimens are processed and slides are prepared using the ThinPrep 2000 processor. Specimens are then stained using a Papanicolaou stain and analyzed microscopically by a cytotechnologist and pathologist.
For FISH analysis, slides prepared from esophageal brushing specimens are assessed by FISH for the presence of cells that have chromosomal abnormalities consistent with a diagnosis of malignancy.(Barr Fritcher EG, Brankley SM, Kipp BR, et al: A comparison of conventional cytology, DNA ploidy analysis, and fluorescence in situ hybridization for the detection of dysplasia and adenocarcinoma in patients with Barrett's esophagus. Human Pathology 2008;39:1128-1135)