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Unit Code 200518:
IgG Subclasses, Serum

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Method Description

The concentrations of IgG subclass proteins in serum are measured

by automated nephelometry in which the concentrations of each protein

are determined from standard curves. The intensity of light scattered

by immune complexes of IgG-subclass proteins and specific reagent

antibodies is compared to standard curves plotted from the reactions

of protein standards with the same reagent antibodies. (Package insert:  Behring Nephelometer II Operations Instruction Manual. Dade Behring, Inc., Newark, DE; Vlug A, Nieuwenhuys EJ, van Eijk RVW, et al: Nephelometric measurements of human IgG subclasses and their reference ranges. Ann Biol Clin 1994;52:561-567)

Performing Laboratory Location

New England

Key