Unit Code 200271:
B-Type Natriuretic Peptide (BNP), Plasma
Method Description
The instrument used is a Beckman Coulter UniCel DxI 800. The BNP
test is a 2-site immunoenzymatic sandwich assay. A sample is added
to a reaction vessel with mouse monoclonal antihuman BNP antibody
alkaline phosphatase conjugate and paramagnetic particles coated
with mouse monoclonal antihuman BNP antibody. BNP in human
plasma binds to the immobilized anti-BNP on the solid phase, while
the mouse anti-BNP conjugate reacts specifically with bound BNP.
After incubation in a reaction vessel, separation in a magnetic field
and washing remove materials not bound to the solid phase. The
chemiluminescent substrate Lumi-Phos* 530 is added to the reaction
vessel and light generated by the reaction is measured. The light
production is directly proportional to the concentration of BNP in the
sample. The amount of analyte in the sample is determined from
a stored, multipoint calibration curve. (Package insert: Biosite
Incorporated, France 2004)
Performing Laboratory Location
New England
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