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Unit Code 200177:
T3 (Triiodothyronine), Free, Serum

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Method Description

The instrument used is a Beckman Coulter DxI 800. The Access

Free T3 assay is a competitive-binding immunoenzymatic Assay.

A sample is added to a reaction vessel with an anti-T3 monoclonal

antibody conjugated to alkaline phosphatase. During the incubation,

free T3 in the sample reacts with the anti-T3 antibody. Particles

coated with streptavidin and biotinylated T3 analog are then added

to the mixture. Unoccupied binding sites on the anti-T3 antibody are

bridged to the particle through the T3 analog. After incubation in a

reaction vessel, materials bound to the solid phase are held in a

magnetic field, while unbound materials are washed away.

Chemiluminescent substrate Lumi-Phos 530 is added to the vessel

and the light generated by the reaction is measured with a luminometer.

The light production is inversely proportional to the concentration of

free T3 in the sample. The amount of analyte in the sample is

determined from a stored, multipoint calibration curve.

(Beckman Coulter Assay Manual 2007)

Performing Laboratory Location

Rochester

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