Leukemia/Lymphoma Immunophenotyping by Flow Cytometry, Tissue
Method Description Describes how the test is performed and provides a method-specific reference
Flow cytometric immunophenotyping of tissues is performed using the following antibodies:
Tissue Panel: CD3, CD5, CD7, CD10, CD19, CD20, CD23, CD45, 7-AAD, and kappa and lambda light chains.
Possible additional panels:
B-cell Panel: CD5, CD10, CD11c, CD19, CD20, CD22, CD38, CD45, CD103, CD200 and kappa and lambda light chains
T-cell Panel: CD2, CD3, CD4, CD5, CD7, CD8, CD26, CD45, and gamma/delta
KIR Panel: CD3, CD8, CD16, CD56, CD57, CD94, CD158a, CD158b, CD158e (p70) and NKG2a
V-Beta Panel: CD3, CD8, T-cell receptors: VB1, VB2, VB3, VB4, VB5.1, VB5.2, VB5.3, VB7.1, VB7.2, VB8, VB9, VB11, VB12, VB13.1, VB13.2, VB13.6, VB14, VB16, VB17, VB18, VB20, VB21.3, VB22, and VB23.
Acute Panel: CD2, CD3, CD5, CD7, CD13, CD15, CD19, CD20, CD33, CD34, CD45, CD56, CD117 and HLA-DR
B-cell ALL, minimal residual disease (MRD) panel: CD9, CD10, CD19, CD20, CD34, CD38, CD45, CD66c
Myeloperoxidase (MPO)/terminal deoxynucleotidyl transferase (TdT) (MPO/TdT) Panel: cytoplasmic CD3, CD13, cytoplasmic CD22, CD34, CD45, cytoplasmic CD79a, nuclear TDT, and cytoplasmic MPO
Plasma Cell Panel: CD19, CD38, CD45, CD138, and cytoplasmic kappa and lambda light chains
(Flow Cytometry in Clinical Diagnosis. Fourth edition. Edited by P Keren, JP Jr McCoy, J Carey. ASCP Press, Chicago, IL, 2007)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Specimens are processed and reported Monday through Saturday
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Remaining tissue 7 days
Performing Laboratory Location The location of the laboratory that performs the test