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Unit Code 15867:
Insulin-Like Growth Factor 1, Serum

Method Description Describes how the test is performed and provides a method-specific reference

IMMULITE 2000 IGF-1 is a solid-phase, chemiluminescent immuno-

metric assay. First, sample pretreatment is accomplished in an

onboard dilution step. Then, patient sample and alkaline

phosphatase-conjugated anti-IGF-1 antibodies are simultaneously

incubated with antibody-coated beads. During this time, IGF-1 in

the sample forms an antibody sandwich complex that binds to the

streptavidin on the beads. Unbound enzyme conjugate is then

removed by washing, after which substrate is added. The

chemiluminescent substrate, a phosphate ester of adamantyl

dioxetane, undergoes hydrolysis in the presence of alkaline

phosphatase to yield an unstable intermediate. The continuous

production of this intermediate results in the sustained emission

of light. The photon output is directly proportional to the

concentration of the IGF-1 in the specimen.

(Package insert:  IMMULITE 2000 IGF-1, Diagnostic Products

Corporation, Los Angeles, CA PIL2KGF-14, 2006-12-29)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 5 a.m.-12a.m., Saturday; 6 a.m.-6 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester