|Values are valid only on day of printing.|
Patient specimen is deposited on the slide where the spreading layer promotes the uniform distribution of the specimen and permits an even penetration of solute molecules into the underlying reagent layer. The oxidation of specimen glucose is catalyzed by glucose oxidase to form hydrogen peroxide and gluconate. The reaction is followed by an oxidative coupling catalyzed by peroxidase in the presence of dye precursors to produce a dye. The intensity of the dye is measured by reflected light.
Each mole of glucose oxidized results in 0.5 mole of dye, assuming quantitative conversions. The intensity of the dye is measured by reflected light at 540 nm and concentration is reported in milligrams per deciliter.(Package insert: Ortho-Clinical Diagnostics. United Kingdom, 2004)
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