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Test ID: HCVPS    
Hepatitis C Antibody Screen Patient Source, Serum

Method Description Describes how the test is performed and provides a method-specific reference

The VITROS anti-hepatitis C virus (HCV) assay is performed using the VITROS Anti-HCV Reagent Pack and VITROS Immunodiagnostic Products Anti-HCV Calibrator on the VITROS Immunodiagnostic System. An immunometric technique is used, involving a 2-stage reaction. In the first stage, hepatitis C virus (HCV) antibody present in the sample binds to HCV recombinant antigens coated on the reaction wells, and unbound sample is removed by washing. In the second stage, horseradish peroxidase (HRP)-labeled antibody conjugate (mouse monoclonal antihuman IgG) binds to human IgG captured on the well in the first stage. Unbound conjugate is removed by washing. A reagent containing luminogenic substrates (a luminal derivative and a peracid salt) and an electron transfer agent is added to the wells. The HRP in the bound conjugate catalyzes the oxidation of the luminal derivative, producing light. The electron transfer agent increases the level and duration of the light produced. The emitted light signals are detected and measured by the VITROS Immunodiagnostic System. The amount of HRP conjugate bound is directly proportional to the level of anti-HCV antibodies present in a given sample.(Package insert: VITROS Anti-HCV Reagent Pack, no. GEM1243, version 8.0, Ortho-Clinical Diagnostics, Inc. Rochester, NY 14626-5101, 1/11/2013)


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