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Test ID: CENTA    
Centromere Antibodies, Serum

Method Description Describes how the test is performed and provides a method-specific reference

In this assay, autoantibodies in a test sample bind to antigens in the substrate (human epithelial cells, Hep-2). Washing removes excess serum from the substrate. Fluorescein conjugated (FITC) antiserum added to the substrate attaches to the bound autoantibody. After a second washing step to remove excess conjugate, the substrate is coverslipped and viewed for fluorescent patterns with a fluorescent microscope. Observation of a specific pattern on the substrate indicates the presence of autoantibodies in the test sample. (Zeus Scientific ANA/Hep-2 Cell Culture IFA Test System Package Insert, Product Series 2400/24000. Issued April 27, 2004)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday, Sunday 3rd shift

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day /2 days if positive

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

2 weeks

Performing Laboratory Location The location of the laboratory that performs the test

New England