JAKXB - Overview: JAK2 Exon 12 and Other Non-V617F Mutation Detection, Blood

Test Catalog

Test Name

Test ID: JAKXB    
JAK2 Exon 12 and Other Non-V617F Mutation Detection, Blood

Useful For Suggests clinical disorders or settings where the test may be helpful

Aiding in the distinction between a reactive cytosis and a myeloproliferative neoplasm, particularly when a diagnosis of polycythemia is being entertained; for use with blood specimens

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

This is a second-order test that should be used when the test for the JAK2B / JAK2 V617F Mutation Detection, Blood test is negative. The sensitivity of this assay is much less than that of the JAK2B blood test. This is because the sequencing technique is required to evaluate for many potential mutations. The sensitive JAK2B blood test should always be performed first, as the JAK2 mutation burden may be very low in some specimens. If JAK2B blood test is negative, then this assay (JAKXB / JAK2 Exon 12 and Other Non-V617F Mutation Detection, Blood) should be performed for detection of non-V617F JAK2 mutations.


The following algorithms are available in Special Instructions:

-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation

-Erythrocytosis Evaluation Testing Algorithm

Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test

Method Name A short description of the method used to perform the test

Mutation Detection in cDNA Using Sanger Sequencing
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

JAK2 Exon 12 Mutation Detection, B

Aliases Lists additional common names for a test, as an aid in searching

JAK2 Exon 12
JAK2 Exon 13
JAK2 Exon 14
JAK2 Exon 15
Janus Kinase 2 Gene
Janus Kinase 2 Gene Sequencing
Tyrosine Kinase Gene Sequencing
Tyrosine Kinase Mutuation