Test ID: BRAF
BRAF Mutation Analysis (V600E), Tumor
Secondary ID 
A test code used for billing and in test definitions created prior to November 2011
Useful For Suggests clinical disorders or settings where the test may be helpful
As an adjunct to HNPCC/17073 Hereditary Nonpolyposis Colorectal Cancer (HNPCC) Screen, when colon tumor demonstrates microsatellite instability (MSI-H) and loss of MLH1 protein expression, to help distinguish a somatic versus germline event prior to performing expensive germline testing
As an adjunct to negative MLH1 germline testing in cases where colon tumor demonstrates MSI-H and loss of MLH1 protein expression
Note: Mayo's preferred screening test (MLBRF/87931 MLH1 Hypermethylation and BRAF Mutation Analyses) includes both MLH1 promoter hypermethylation and BRAF V600E testing. Please note that test can only be performed on colon tumors.
To identify colon tumors with a previously negative KRAS mutation analysis result that may respond to EGFR-targeted therapies
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
See Hereditary Nonpolyposis Colorectal Cancer Testing Algorithm in Special Instructions.
Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test
Method Name A short description of the method used to perform the test
Polymerase Chain Reaction (PCR) Analysis
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)
External
link
PDF
document
Excel
document
Word
document