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Test Catalog

Test ID: MLBRF    
MLH1 Hypermethylation and BRAF Mutation Analyses, Tumor

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Useful For Suggests clinical disorders or settings where the test may be helpful

As an adjunct to HNPCC / Hereditary Nonpolyposis Colorectal Cancer (HNPCC) Screen, when colon tumor demonstrates microsatellite instability (MSI-H) and loss of MLH1 protein expression, to help distinguish a somatic versus germline event prior to performing expensive germline testing


As an adjunct to negative MLH1 germline testing in cases where colon tumor from the same patient demonstrates MSI-H and loss of MLH1 protein expression

Genetics Test Information Provides information that may help with selection of the correct test or proper submission of the test request

If this test is ordered in conjunction with the HNPCC screen (HNPCC) or MLH1 immunostain (IHCO) and MSI (MSIO), this test (MLBRF) will only be performed when clinically indicated.

Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test IDReporting NameAvailable SeparatelyAlways Performed
MLHHMMLH1 HypermethylationYes, (order MLH1H)Yes
BRAFPBRAF Mutation Analysis (V600E)Yes, (order BRAF)Yes
MBPINMLH1 Hyp/BRAF (V600E) Mut InterpNoYes

Additional Tests Lists test(s) that are always performed, at an additional charge, with the initial test(s)

Test IDReporting NameAvailable SeparatelyAlways Performed
SLRVWSlide ReviewNoYes

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, slide review will always be performed at an additional charge.


See Hereditary Nonpolyposis Colorectal Cancer Testing Algorithm in Special Instructions.

Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test

Method Name A short description of the method used to perform the test

Polymerase Chain Reaction (PCR) Analysis

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

MLH1 Hypermethylation/BRAF Mutation

Aliases Lists additional common names for a test, as an aid in searching

BRAF Mutation
MLH1 Hypermethylation
Promoter Hypermethylation