ESTF - Overview: Estrogens, Estrone (E1) and Estradiol (E2), Fractionated, Serum

Test Catalog

Test ID: ESTF    
Estrogens, Estrone (E1) and Estradiol (E2), Fractionated, Serum

Useful For Suggests clinical disorders or settings where the test may be helpful

This test allows the simultaneous high-sensitivity determination of serum estrone and estradiol levels. It is useful in situations requiring either higher sensitivity estradiol measurement, or estrone measurement, or both. This includes the following:

-As part of the diagnosis and workup of precocious and delayed puberty in females, and, to a lesser degree, males

-As part of the diagnosis and workup of suspected disorders of sex steroid metabolism, eg, aromatase deficiency and 17 alpha-hydroxylase deficiency

-As an adjunct to clinical assessment, imaging studies, and bone mineral density measurement in the fracture risk assessment of postmenopausal women, and, to a lesser degree, older men

-Monitoring low-dose female hormone replacement therapy in postmenopausal women

-Monitoring antiestrogen therapy (eg, aromatase inhibitor therapy)


Useful in all applications that require moderately sensitive measurement of estradiol including:

-Evaluation of hypogonadism and oligo-amenorrhea in females

-Assessing ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)


In conjunction with luteinizing hormone measurements, monitoring of estrogen replacement therapy in hypogonadal premenopausal women


Evaluation of feminization, including gynecomastia, in males


Diagnosis of estrogen-producing neoplasms in males, and, to a lesser degree, females

Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test IDReporting NameAvailable SeparatelyAlways Performed
E1Estrone, SYesYes
EESTEstradiol, Mass Spectrometry, SYesYes

Method Name A short description of the method used to perform the test

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

Estrogens, E1+E2, fractionated, S

Aliases Lists additional common names for a test, as an aid in searching

E1 (Estrone)
Estrogens, total
Total Estrogens