70064 - Overview: Renal Pathology Consultation, Wet Tissue

Test Catalog

Test Name

Test ID: 70064    
Renal Pathology Consultation, Wet Tissue

Useful For Suggests clinical disorders or settings where the test may be helpful

The evaluation and management of patients with kidney disease


Following the progression of known renal disease and/or response to therapy


Determining the cause of dysfunction in the transplanted kidney (allograft)

Reflex Tests Lists test(s) that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial test(s)

Test IDReporting NameAvailable SeparatelyAlways Performed
4986Renal Bx, Electron MicroscopyNoNo
20500RenalPath, Level IV, Wet TsNoNo
9840Periodic Acid-Schiff (PAS) STNoNo
9828Masson's Trichrome ST for Conn TsNoNo
9889Jones' Methenamine Silver STNoNo
84226Immunofluorescent C4d StainNoNo
60301IgA IF, RenalNoNo
60302IgG IF, RenalNoNo
60304Lambda IF, RenalNoNo
60305Kappa IF, RenalNoNo
60306C1q IF, RenalNoNo
60307C3 IF, RenalNoNo
60308Albumin IF, RenalNoNo
60309Fibrinogen IF, RenalNoNo
60328IgG1 IF, RenalNoNo
60329IgG2 IF, RenalNoNo
60330IgG3 IF, RenalNoNo
60331IgG4 IF, RenalNoNo
60285C1q Paraffin IF, RenalNoNo
60287IgA Paraffin IF, RenalNoNo
60288IgG Paraffin IF, RenalNoNo
60289IgM Paraffin IF, RenalNoNo
60290Kappa Paraffin IF, RenalNoNo
60291Lambda Paraffin IF, RenalNoNo
60303IgM IF, RenalNoNo
62196RenalPath Consult, Outside SlideNo, (Bill Only)No
62197RenalPath Consult, w/Slide PrepNo, (Bill Only)No
62198RenalPath Consult, w/Comp Rvw of HisNo, (Bill Only)No

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

The Renal Pathology Consultation is performed by a Mayo Clinic renal pathologist and entails the performance of appropriate procedures and stains based on the material received, patient information and specific findings on the case in order to determine a diagnosis. Optimal/standard diagnostic interpretation of a medical kidney biopsy requires integration of the light microscopy, immunofluorescence, and electron microscopy findings together with the clinical and laboratory data for the patient. We follow published standards by the Renal Pathology Society  for the diagnostic evaluation and reporting of non-neoplastic renal disease.(1)


Light Microscopy:

Wet/unprocessed tissue submitted for light microscopy routinely includes the preparation of stains, which include hematoxylin and eosin (H and E), periodic acid Schiff (PAS), Masson trichrome, and Jones methenamine silver stains. 



Wet/unprocessed tissue submitted for immunofluorescence may include the following stains in order to render an accurate diagnosis. These stains include: IgA, IgG, IgM, C1q, C3, albumin, fibrinogen, kappa light chain, and lambda light chain stains with C4d added if the biopsy is an allograft.


The IgG subtypes (IgG1, IgG2, IgG3, IgG4) are typically only utilized if the biopsy shows features suspicious for a monoclonal/monotypic deposition process involving IgG.


Alport (collagen IV, alpha 2 and alpha 5) staining is performed in the setting of biopsy findings that are consistent with hereditary nephritis/Alport syndrome.


Paraffin-Based Immunofluorescence Stains:

The paraffin-based immunofluorescence stains listed above would only be utilized in the special circumstance when there is no tissue or inadequate tissue available for standard immunofluorescence or if there are findings that raise concern for so-called “masked” deposits.


Phospholipase A2 Receptor (PLA2R) staining is performed in the setting of membranous nephropathy/glomerulonephritis to aid in determining whether it is most likely primary/idiopathic or secondary.


Electron Microscopy:

Wet/unprocessed tissue submitted for electron microscopy will be processed for transmission electron microscopy. A formal interpretive report is issued, incorporating the findings from all tests performed for diagnostic purposes.


See Pathology Consultation Ordering Algorithm in Special Instructions.

Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test

Method Name A short description of the method used to perform the test

Pathology Consultation

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

RenalPath Consultation, Wet Tissue

Aliases Lists additional common names for a test, as an aid in searching

Kidney Allograft Biopsy
Kidney Biopsy
Kidney Transplant Biopsy
Renal Allograft Biopsy
Renal Biopsy
Renal Transplant Biopsy