LLPT - Overview: Leukemia/Lymphoma Immunophenotyping by Flow Cytometry, Tissue

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Test ID: LLPT    
Leukemia/Lymphoma Immunophenotyping by Flow Cytometry, Tissue

Useful For Suggests clinical disorders or settings where the test may be helpful

Evaluation of tissues for potential involvement by:

-Chronic lymphoproliferative disorders

-Malignant lymphomas

-Acute lymphoblastic leukemia

-Acute myelogenous leukemia

Reflex Tests Lists test(s) that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial test(s)

Test IDReporting NameAvailable SeparatelyAlways Performed
FCINTFlow Cytometry Interp, 2-8 MarkersNo, (Bill Only)No
FCIMSFlow Cytometry Interp, 9-15 MarkersNo, (Bill Only)No
FCINSFlow Cytometry Interp,16 or greaterNo, (Bill Only)No

Additional Tests Lists test(s) that are always performed, at an additional charge, with the initial test(s)

Test IDReporting NameAvailable SeparatelyAlways Performed
FIRSTFlow Cytometry, Cell Surface, FirstNo, (Bill Only)Yes
ADD1Flow Cytometry, Cell Surface, AddlNo, (Bill Only)Yes

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, a screening panel and a professional interpretation will always be charged. The screening panel will be charged based on number of makers tested (FIRST for first marker, ADD1 for each additional marker). The interpretation will be set based on markers tested in increments of 9 to 15, or 16 and greater. In addition, reflex testing may occur to fully characterize a disease state or clarify any abnormalities from the screening test. Reflex tests will be performed at an additional charge for each marker tested (FIRST if applicable, ADD1 if applicable).


The tissue panel is initially performed to evaluate for monotypic B-cells by kappa and lambda light chain expression, and increased numbers of blasts and plasma cells by CD45 expression along with side scatter gating. The panel can also evaluate T cells with CD3, CD5, and CD7. Additionally, viability is assessed on all tissue specimens using 7-AAD exclusion.


This panel, together with the provided clinical history and morphologic review, is used to determine what, if any, further testing is needed for disease diagnosis or classification. If additional testing is required, it will be added per algorithm to fully characterize a disease state with a charge per unique antibody tested.


In addition to reflexing flow cytometric panels, FISH or molecular testing may be recommended by the Mayo pathologist to facilitate diagnosis. They will contact the referring physician or pathologist to confirm the addition of these tests.

Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test

Method Name A short description of the method used to perform the test


NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

Leukemia Lymphoma Phenotype, Tissue

Aliases Lists additional common names for a test, as an aid in searching

Flow Cytometry, Tissue
Leukemia Immunophenotyping, Tissue Panel
Lymphoma Immunophenotyping by Flow Cytometry
Lymphoma Immunophenotyping, Tissue
Tissue Panel, Leukemia Immunophenotyping