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Test ID: VWAG    
von Willebrand Factor Antigen, Plasma

Useful For Suggests clinical disorders or settings where the test may be helpful

von Willebrand factor (VWF) antigen measurement is most effective when it is combined with measurement of VWF ristocetin cofactor activity and factor VIII coagulant activity, preferably as a panel of tests with reflexive testing and interpretive reporting. Within this context, VWF antigen measurement can be useful for:

-Diagnosis of von Willebrand disease (VWD) and differentiation of VWD subtype

-Differentiation of VWD from hemophilia A (in conjunction with factor VIII coagulant assay)

 

Monitoring therapeutic efficacy of treatment with DDAVP (desmopressin) or VWF concentrates in patients with VWD

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

The von Willebrand factor (VWF) is a multimeric adhesive glycoprotein that is important for platelet-platelet and platelet-vessel hemostatic interactions. In addition, plasma VWF serves as a carrier protein for coagulation factor VIII, stabilizing its procoagulant activity. VWF circulates in the blood in 2 distinct compartments; plasma VWF mainly reflects VWF synthesis and release from vascular endothelial cells, and platelet VWF (about 10% of the blood VWF) reflects VWF synthesis by bone marrow megakaryocytes with storage primarily in the alpha granules of circulating platelets. VWF antigen measurement assesses the mass of plasma VWF protein, but does not reflect VWF functions or platelet VWF. The major function of VWF (mediating platelet-platelet or platelet-vessel interaction) is most commonly assessed by measurement of plasma ristocetin cofactor activity.

 

Decreased VWF antigen may be seen in:

-Congenital von Willebrand disease

-Acquired VWD that may be associated with monoclonal gammopathies, lymphoproliferative disorders, autoimmune disorders, and hypothyroidism

 

Increased VWF antigen may be seen in association with:

-Pregnancy and/or estrogen use

-Inflammation (acute-phase reactant)

-Exercise or stress

-Liver disease

-Vasculitis

-Thrombotic thrombocytopenic purpura/hemolytic uremic syndrome

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

55-200%

Note: Individuals of blood group "O" may have lower plasma von Willebrand factor (VWF) antigen than those of other ABO blood groups, such that apparently normal individuals of blood group "O" may have plasma VWF antigen as low as 40% to 50%, whereas the lower limit of the reference range for individuals of other blood groups may be 60% to 70%.

Children: Neonates, infants, and children have normal or mildly increased plasma VWF antigen, with respect to the adult reference range.

Interpretation Provides information to assist in interpretation of the test results

von Willebrand factor (VWF) antigen assay results generally must be used together with assays of VWF ristocetin cofactor activity and factor VIII coagulant activity, for optimum clinical utility and diagnostic efficiency. The diagnosis of von Willebrand disease (VWD) requires a combination of clinical and laboratory information. We suggest ordering VWPR / von Willebrand Profile.

 

Patients with congenital severe type III VWD have a markedly decreased or undetectable level of VWF antigen in the plasma (and in the platelets), in addition to a plasma ristocetin cofactor activity that is very low, or not detectable.

 

Patients with types IIA and IIB variants of VWF (with abnormal plasma VWF function and multimeric structure) may have normal or decreased plasma VWF antigen. However, they typically have decreased plasma ristocetin cofactor activity, along with decreased higher molecular-weight VWF multimers in the plasma.

 

Patients with types IIM or IIN VWD have normal levels of VWF antigen. In spite of this, they either have decreased vWF ristocetin cofactor activity, not caused by absence of higher molecular weight vWF multimers (type IIM VWD), or decreased factor VIII coagulant activity (type IIN VWD)

 

Patients with type I VWD (with decreased but normally functioning plasma VWF) have concordantly decreased plasma VWF antigen and ristocetin cofactor activity.

 

Patients with acquired VWD may have either normal or decreased plasma VWF antigen.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Lipemic specimens may lead to an underestimation of the von Willebrand factor (VWF) level.

 

The presence of rheumatoid factor may lead to an overestimation of the VWF level.

 

VWF is an acute-phase reactant and may be elevated above baseline in association with a variety of conditions including inflammation, stress, exercise, liver disease, pregnancy or estrogen therapy. Baseline VWF levels also increase with aging. These conditions may obscure diagnosis of the milder forms of von Willebrand disease (VWD). Repeat testing may be indicated.

 

Low normal levels of VWF antigen do not exclude possible diagnosis of VWD.

 

Borderline low or slightly decreased levels of VWF antigen may be observed in clinically normal individuals of blood group "O."

Supportive Data

Mayo studies demonstrate excellent concordance between the enzyme-linked immunosorbent assay and this automated latex immunoassay (LIA) (r[2]=0.95) in about 80 patient specimens (with von Willebrand factor [VWF] antigen ranging from 3% to 800%), and satisfactory concordance between VWF antigen by LIA and ristocetin cofactor activity (r[2]=0.88).

Clinical Reference Provides recommendations for further in-depth reading of a clinical nature

1. Sadler JE, Blinder M: von Willebrand disease: diagnosis, classification and treatment. In Hemostasis and Thrombosis: Basic Principles and Clinical Practice. Fifth edition. Edited by RW Colman, VJ Marder, AW Clowes, et al. Baltimore, MD, Lippincott Williams and Wilkins, 2006, pp 905-921

2. Eby C, Chance D, Oliver D: A multicenter evaluation of ATA-LIATEST VWF: A new latex particle immunoassay for von Willebrand factor antigen. Clin Hemostasis Rev 1997;11:16-17

3. Rodeghiero F, Castaman G, Tosetto A: Von Willebrand factor antigen is less sensitive then ristocetin cofactor for the diagnosis of type K von Willebrand disease – Results based on an epidemiological investigation. Thromb Haemost 1990;64:349-352

4. Triplett DA: Laboratory diagnosis of von Willebrand’s disease. Mayo Clin Proc 1991;66:832-840

Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test