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Test ID: FB12V    
Vitamin B12 Deficiency Panel

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

INTERPRETATION:

                    NORMAL     B12 DEFICIENCY     FOLATE DEFICIENCY

Metabolite    %HIGH                %HIGH                                %HIGH

           RANGE VALUES   RANGE   VALUES      RANGE    VALUES

Methylmalonic Acid:

                      73-271   <3            271-200,000  >95             73-271    <3

2-Methylcitric Acid:

                      60-228   <3             228-15,000   >80              60-228    <3

Homocysteine:

                      5.1-13.9 <3                 14-500       >95             14-250    >95

Cystathionine:

                       44-342  <3             342-4000      >80         342-18,000 >80

    

NOTE 1)Serum Methylmalonic Acid and Homocysteine are the primary metabolic tests for diagnosing and distinguishing between B12 and folate deficiency. They can be used in conjunction with the serum B12 which is usually low or low normal (<350 pg/mL) in B12 deficiency and the serum folate which is usually low or low normal (<5 ng/mL) in folate deficiency. 2-Methylcitric Acid and Cystathionine provide confirmatory evidence for such deficiencies. Homocysteine and especially Cystathionine may also be high in B6 deficiency.

    

NOTE 2) Elevated levels of serum metabolites will correct to normal after treatment with the appropriate vitamin but will not correct after treatment with the wrong vitamin, even in pharmacologic amounts.

    

NOTE 3) Any of the four metabolites can be elevated due to renal insufficiency or intravascular volume depletion. This occurs most commonly in the case of 2-Methylcitric Acid and Cystathionine. Elevated metabolite levels do not correct with B12, folate or B6 treatment unless vitamin deficiency coexists.

 

NOTE 4) Serum metabolite levels can be rechecked 5 to 15 days after vitamin therapy.

    

NOTE 5) Normal ranges 6 hours post oral Methionine load (100 mg L-Methionine/kg body wt.) are as follows: Homocysteine 16.5-45.7 umoles/Liter and Cystathionine 424-2500 nmoles/Liter. Methylmalonic Acid and 2-Methylcitric Acid do not change after a Methionine load.

    

Test Performed by: Metabolite Laboratories, Inc.

                             Department of Hematology

                             University of Colorado Denver

                             12700 E. 19th Ave., Room 9122/9125

                             Building P-15, Research Complex 2

                             Aurora, CO 80045