|Values are valid only on day of printing.|
Detection of allo- or autoantibodies directed against red blood cell antigens in the settings of pretransfusion testing
Evaluation of transfusion reactions
Evaluation of hemolytic anemia
If the antibody screen is positive, then antibody identification will be performed.
The following tests may also be ordered and performed as part of antibody identification: monospecific direct Coombs, special red cell antigen typing, antibody elution, and antibody absorption.
Transfusion and pregnancy are the primary means of sensitization to red cell antigens.
In a given population, 2% to 4% of the general population possess irregular red cell alloantibodies. Such antibodies may cause hemolytic disease of the newborn or hemolysis of transfused donor red blood cells.
Autoantibodies react against the patient's own red cells as well as the majority of cells tested. Autoantibodies can be clinically benign or can hemolyze the patient's own red blood cells, such as in cold agglutinin disease or autoimmune hemoyltic anemia.
If positive, antibody identification will be performed.
A positive result (antibody detected) necessitates antibody identification to establish the specificity and clinical significance of the antibody detected.
Negative–no antibody detected.
Clinical evaluation of antibodies identified is necessary to determine their potential for harm to the patient at this time and to assess appropriate action to be taken in the future.
Technical Manual. 14th edition. Edited by RH Walker. Bethesda, MD, American Association of Blood Banks, 2002, pp 379-418