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Determining class I HLA antigens on specimens for transplant candidates and their donors or those who have become refractory to platelet transfusions
Human leukocyte antigens (HLA) are regulators of the immune response that play a key role in transplantation. HLA class I typing is most frequently applicable to organ transplant donor/recipient matching, provision of HLA-matched platelets for alloimmunized refractory patients, and for a small number of disease associations.
Class I HLA antigens include A, B, and C loci. This assay is designed to provide low-to-medium resolution for HLA class I typing (A, B, C). Low-to-medium resolution defines the typing at the antigen level. This is in contrast to high-resolution typing, which defines typing at the allele (molecular) level and is used primarily for typing donor/recipient pairs for unrelated bone marrow transplantation.
Interpretation depends on the rationale for ordering the test. Assessments of acceptable donor/recipient matches are made on a case-by-case basis.
No significant cautionary statements
1. Terasaki PI, Bernoco D, Park MS, et al: Microdroplet testing for HLA-A, B, C and D antigens. Am J Clin Pathol 1978 Feb;69(2):103-120
2. Colinas RJ, Bellisario R, Pass KA: Multiplexed genotyping of beta-globin variants form PCR-amplified newborn blood spot DNA by hybridization with allele-specific oligodeoxynucleotides coupled to an array of fluorescent microspheres. Clin Chem 2000 Jul;46(7):996-998