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Unit Code 88897:
B-Cell Lymphoma, FISH, Blood or Bone Marrow

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Useful For

Detecting an abnormal clone associated with the common chromosome

anomalies seen in patients with B-cell lymphoma, specifically Burkitt,

mantle cell, follicular, diffuse large B-cell, and MALT lymphoma

 

Individual probes can also be utilized to identify specific chromosome

anomalies in patients with B-cell lymphoma and track the response

to therapy.

Clinical Information

Lymphoid neoplasms are known to be complex and the prognosis

and clinical course of patients with lymphoma is highly variable.

Genetic abnormalities have emerged as 1 of the most reliable

criteria for categorizing lymphomas. Several chromosome anomalies

and variants of these anomalies have been associated with various

kinds of lymphoma (see Table).

 

Common chromosome anomalies in lymphomas

 

Lymphoma Type

Chromosome anomaly

FISH Probe

Burkitt

t(8;14)(q24;q32)

c-MYC/IGH

Mantle cell

t(11;14)(q13;q32)

CCND1-XT/IGH

Follicular

t(14;18)(q32;q21)

IGH/BCL2

Diffuse large B-cell

t(3;var)(q27;var)

BCL6

t(14;18)(q32;q21)

IGH/BCL2

t(8;14)(q24;q32)

c-MYC/IGH

Marginal zone of

MALT type

t(11;18)(q21;q21)

API2/MALT1

t(14;18)(q32;q21)

IGH/MALT1

 

Reference Values

An interpretive report will be provided.

Interpretation

A neoplastic clone is detected when the percent of cells with an

abnormality exceeds the normal reference range for any given probe.

 

The absence of an abnormal clone does not rule the presence of

neoplastic disorder.

Cautions

This test is not approved by the Food and Drug Administration (FDA)

and it is best used as an adjunct to existing clinical and pathologic

information.

Special Instructions and Forms

• Cytogenetics Hematologic FISH Panel Patient Information Sheet 

Clinical Reference

1.   Remstein ED, Kurtin PJ, Buno I, et al:  Diagnostic utility of fluorescence

      in situ hybridization in mantle-cell lymphoma. Br J Haematol 2000 Sep;

      110(4):856-862

 

2.   Remstein ED, Kurtin PJ, James CD, et al:  Mucosa-associated

      lymphoid tissue lymphomas with t(11;18) (q21;q21) and mucosa-

      associated lymphoid tissue lymphomas with Aneuploidy develop

      along different pathogenetic pathways. Am J Pathol 2002 Jul;161(1):

      63-71

 

3.   Remstein ED, Dogan A, Einerson RR, et al:  The incidence and

      anatomic site specificity of chromosomal translocations in primary

      extranodal marginal zone B-cell lymphoma of mucosa-associated

      lymphoid tissue (MALT lymphoma) in North America. Am J Surg

      Pathol 2006 Dec;30(12):1546-1553H636

 

Key