POXA - Clinical: Oxalate, Plasma

Test Catalog

Take Our Survey

Test ID: POXA    
Oxalate, Plasma

Useful For Suggests clinical disorders or settings where the test may be helpful

Assessing the body pool size of oxalate. The settings in which it has been most useful include patients with enzyme deficiencies, such as primary hyperoxaluria, which result in overproduction of oxalate or patients with enteric hyperoxaluria (EH).

 

In the presence of chronic kidney disease (CKD), 3 uses of plasma oxalate are:

 

-If primary hyperoxaluria (PH) is suspected in a patient with CKD of indeterminate cause, and urinary oxalate is not available, plasma oxalate can be used to aid in diagnosis. However although  plasma oxalate levels are markedly elevated in PH patients with CKD suggesting the diagnosis, ancillary tests often are necessary to confirm it, such as genetic analysis of the 3 causative genes, or pathologic demonstration of oxalate crystals in tissues

-Monitoring patients with renal failure and primary or enteric hyperoxaluria in order to be sure they are receiving enough dialysis

-An aid in maintaining plasma oxalate levels below supersaturation (25-30 mcmol/L)

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

See Hyperoxaluria Diagnostic Algorithm in Special Instructions.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Oxalate is an insoluble dicarboxylic acid, which is an end product of liver metabolism of glyoxalate and glycerate. Humans lack an enzyme to degrade oxalate, and thus it must be eliminated by the kidney. Oxalate is a strong anion and tends to precipitate with calcium, especially in the urinary tract. Consequently, about 75% of all kidney stones contain calcium oxalate in some proportion. In renal failure oxalate is retained in the body and it can precipitate in tissues causing tissue toxicity, a condition called oxalosis.

 

In the absence of disease, up to 90% of the body pool of oxalate is produced by hepatic metabolism and the other 10% is provided by oxalate contained in various foods. However, in the presence of gastrointestinal diseases that cause fat malabsorption the percentage absorbed from food can be much greater. The oxalate content of fruits and vegetables is quite variable, some being quite high and others virtually zero.

 

Oxalate is freely filtered by the glomerulus. A smaller amount is also secreted in the proximal tubule. If the glomerular filtration rate (GFR) is decreased, oxalate begins to be retained in the body. However, in persons without primary hyperoxaluria (PH) or enteric hyperoxaluria (EH) plasma levels do not exceed the normal range until the GFR decreases below 10-20 mL/min/1.73 m(2).

 

Plasma oxalate concentration is a reflection of the body pool size. When the pool increases, oxalate may precipitate in tissues and cause toxicity. Plasma oxalate pool size can be increased in various situations:

 

Increased production and accumulation results from an abnormality in at least 3 different enzymes:

 

Alanine glyoxalate transferase is necessary for the conversion of glycolate to alanine. A deficiency or intracellular mistargeting of this hepatic enzyme results in increased oxalate production (primary hyperoxaluria type1).

 

Glycolate reductase / hydroxypyruvate reductase deficiency in the liver and elsewhere in the body results in increased glyceric acid formation, which leads to increased oxalate production (primary hyperoxaluria type 2).

 

A third type of PH was recently shown to be due to mutations of HOGA1 that encodes the enzyme
4-hydroxy-2-oxaloglutarate aldolase that is found in hepatic mitochondria (primary hyperoxaluria type 3).

 

Increased oxalate load can be caused by increased absorption from the intestines after consuming large amounts of oxalate-rich foods such as rhubarb, spinach, or nuts.

 

Certain abnormalities of the gastrointestinal tract can cause fat malabsorption including short bowel syndromes, inflammatory bowel disease, gastric bypass for obesity, and pancreatic insufficiency. All of these gastrointestinal abnormalities result in increased oxalate absorption from the intestinal tract. This is due to saponification of calcium by fatty acids in the colon, which in turn frees up oxalate anions for absorption.

 

Decreased urinary oxalate excretion in chronic kidney disease (CKD) also caused oxalate retention in the body.

 

Management of patients with PH and renal failure is difficult. Intensive dialyses are undertaken in an attempt to keep plasma levels below the level at which supersaturation and crystallization can occur in body tissues such as heart and bones (called oxalosis).

 

PH is typically diagnosed by measuring oxalate levels in urine. However, as kidney function decreases, the renal excretion of oxalate also decreases. In such situations, plasma oxalate levels may be informative. Although plasma oxalate increases in CKD patients without PH, values are much higher in those CKD patients who do have PH. Plasma oxalate is often used to monitor these patients during critical periods in and around kidney transplantation, dialysis, or liver transplantation.

 

Oxalate concentration in dialysate fluid is a reflection of the oxalate removed during dialysis.

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

<1.6 mcmol/L

Reference values have not been established for patients under 21 and greater than 81 years of age.

Interpretation Provides information to assist in interpretation of the test results

In nonacidified plasma specimens values near the reference range increase an average of 50% due to spontaneous oxalate generation.

 

In patients with normal renal function, the presence of increased plasma oxalate concentration is good evidence for overproduction of oxalate (primary hyperoxaluria).

 

In the presence of renal insufficiency, plasma oxalate levels are markedly elevated. Increased levels of plasma oxalate can be found in dialysis patients.

 

In patients with possible primary hyperoxaluria and renal insufficiency, the diagnosis often can be made by knowing the plasma level of oxalate. However, ancillary tests, such as the demonstration of oxalate crystals in tissues (other than the kidney) or increased glycolate in dialysate (for patients on dialysis) often are necessary to make an accurate diagnosis.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Because increased production and decreased excretion rates of oxalate can increase the plasma oxalate concentration, the interpretation of any given plasma value must consider the patient's clinical setting.

 

Proper specimen processing and acidification are essential to obtain a quality result (see Specimen Required).

 

For external clients only, non-acidified specimens can be accepted if the heparinized plasma is promptly frozen. However, in nonacidified plasma oxalate values may increase spontaneously (average 50% increase for plasma oxalate <15 mcmol/L; average 10% increase for plasma oxalate >15 mcmol/L).

Supportive Data

There is a difference (189%) between the new method (implemented June 2004) and the old method (R[2]=0.9813; y=1.1975x + 1.901).

Clinical Reference Provides recommendations for further in-depth reading of a clinical nature

1. Milliner DS, Eickholt JT, Bergstralh EJ, et al: Results of long-term treatment with orthophosphate and pyridoxine in patients with primary hyperoxaluria. N Engl J Med 1994;331:1553-1558

2. Kuiper JJ: Initial manifestation of primary hyperoxaluria type I in adults--recognition, diagnosis, and management. West J Med 1996;164:42-53

Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test


Key