JC Virus (JCV) Detection by In Situ Hybridization
Confirming a clinical and histopathologic diagnosis of progressive multifocal leukoencephalopathy (PML)
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
JC virus (JCV) is the etiologic agent of progressive multifocal leukoencephalopathy (PML), a rare, demyelinating, fatal disorder of the central nervous system that occurs on a background of immune deficiency. PML is an infrequent complication of a wide variety of conditions, including lymphoproliferative disorders (Hodgkin disease, chronic lymphocytic leukemia), sarcoidosis, tuberculosis, and AIDS.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request 70012 / Pathology Consultation along with this test.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Studies have shown potential cross reactivity between BK virus and JC virus in situ hybridization (ISH) testing.(1)
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Fritzsche FR, Pianca S, Gaspert A, et al: Silver-enhanced in situ hybridization for detection of polyomavirus DNA in patients with BK virus nephropathy. Diagn Mol Pathol 2011;20(2):105-110
2. Aksamit AJ, Mourrain P, Sever JL, Major EO: Progressive multifocal leukoencephalopathy: investigation of three cases using in situ hybridization with JC virus biotinylated DNA probe. Ann Neurol 1985;18:490-496
3. Aksamit AJ Jr: Nonradioactive in situ hybridization in progressive multifocal leukoencephalopathy. Mayo Clin Proc 1993;68:899-910
4. Muaoz-Marmol AM, Mola G, Fernandez-Vasalo A, et al: JC virus early protein detection by immunohistochemistry in progressive multifocal leukoencephalopathy: a comparative study with in situ hybridization and polymerase chain reaction. J Neuropathol Exp Neurol 2004;63(11):1124-1130
5. Vago L, Cinque P, Sala E, et al: JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature. J Acquir Immune Defic Syndr Hum Retrovirol 1996 Jun 1;12(2):139-146