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Test Catalog

Test ID: 81107    
JC Virus (JCV) Detection by In Situ Hybridization

Useful For Suggests clinical disorders or settings where the test may be helpful

Confirming a clinical and histopathologic diagnosis of progressive multifocal leukoencephalopathy (PML)

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

JC virus (JCV) is the etiologic agent of progressive multifocal leukoencephalopathy (PML), a rare, demyelinating, fatal disorder of the central nervous system which occurs on a background of immune deficiency. PML is an infrequent complication of a wide variety of conditions, including lymphoproliferative disorders (Hodgkin disease, chronic lymphocytic leukemia), sarcoidosis, tuberculosis, and immunodeficiency diseases (AIDS).

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.


Interpretation Provides information to assist in interpretation of the test results

This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation/analysis is needed, please request test 70012 / Pathology Consultation along with this test.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Studies have shown potential cross reactivity between BK and JCV ISH testing (Fritzsche FR, Pianca S, Gaspert A, et al: Silver-enhanced in situ hybridization for detection of polyomavirus DNA in patients with BK virus nephropathy. Diagn Mol Pathol. 2011 20(2):105-10).

Clinical Reference Provides recommendations for further in-depth reading of a clinical nature

1. Aksamit AJ, Mourrain P, Sever JL, Major EO: Progressive multifocal leukoencephalopathy: investigation of three cases using in situ hybridization with JC virus biotinylated DNA probe. Ann Neurol 1985;18:490-496

2. Aksamit AJ Jr: Nonradioactive in situ hybridization in progressive multifocal leukoencephalopathy. Mayo Clin Proc 1993;68:899-910

3. Muñoz-Mármol AM, Mola G, Fernández-Vasalo A, et al: JC virus early protein detection by immunohistochemistry in progressive multifocal leukoencephalopathy: a comparative study with in situ hybridization and polymerase chain reaction. J Neuropathol Exp Neurol 2004;63(11):1124-1130

4. Vago L, Cinque P, Sala E, et al: JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature. J Acquir Immune Defic Syndr Hum Retrovirol. 1996 Jun 1;12(2):139-146