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Confirming a clinical and histopathologic diagnosis of progressive multifocal leukoencephalopathy (PML)
JC virus (JCV) is the etiologic agent of progressive multifocal leukoencephalopathy (PML), a rare, demyelinating, fatal disorder of the central nervous system that occurs on a background of immune deficiency. PML is an infrequent complication of a wide variety of conditions, including lymphoproliferative disorders (Hodgkin disease, chronic lymphocytic leukemia), sarcoidosis, tuberculosis, and AIDS.
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request 70012 / Pathology Consultation along with this test.
Studies have shown potential cross reactivity between BK virus and JC virus in situ hybridization (ISH) testing.(1)
1. Fritzsche FR, Pianca S, Gaspert A, et al: Silver-enhanced in situ hybridization for detection of polyomavirus DNA in patients with BK virus nephropathy. Diagn Mol Pathol 2011;20(2):105-110
2. Aksamit AJ, Mourrain P, Sever JL, Major EO: Progressive multifocal leukoencephalopathy: investigation of three cases using in situ hybridization with JC virus biotinylated DNA probe. Ann Neurol 1985;18:490-496
3. Aksamit AJ Jr: Nonradioactive in situ hybridization in progressive multifocal leukoencephalopathy. Mayo Clin Proc 1993;68:899-910
4. Muaoz-Marmol AM, Mola G, Fernandez-Vasalo A, et al: JC virus early protein detection by immunohistochemistry in progressive multifocal leukoencephalopathy: a comparative study with in situ hybridization and polymerase chain reaction. J Neuropathol Exp Neurol 2004;63(11):1124-1130
5. Vago L, Cinque P, Sala E, et al: JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature. J Acquir Immune Defic Syndr Hum Retrovirol 1996 Jun 1;12(2):139-146