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Detecting bacteria responsible for infections of sterile body fluids, tissues, or wounds
Sterile Body Fluids and Normally Sterile Tissues:
In response to infection, fluid may accumulate in any body cavity.
Wound, Abscess, Exudates:
Skin and soft tissue infections can occur as a result of a break in the skin surface, or they can occur as complications of surgery, trauma, human, animal, or insect bites, or diseases that interrupt a mucosal or skin surface. Specimen collection is of utmost importance for these specimen types. For most open lesions and abscesses, remove the superficial flora by decontaminating the skin before collecting a specimen from the advancing margin or base. A closed abscess is the specimen site of choice. Aspirate the abscess contents with a syringe.
No growth or usual flora
Identification of probable pathogens
Any microorganism found where no resident flora is present is considered significant and is reported. For specimens contaminated with the usual bacterial flora, bacteria that are potentially pathogenic are identified.
No significant cautionary statements.
Forbes BA, Sahm DF, Weissfeld AS: Bailey and Scott's Diagnostic Microbiology. 12th edition. Mosby, St. Louis, MO, 2007, Chapters 55, 56, 58, 60,61