Unit Code 80785:
Kappa and Lambda Light Chain Messenger RNA Detection by In Situ Hybridization
Useful For
Detection of clonal proliferation of kappa or lambda light chains
Clinical Information
Kappa and Lambda light chains are expected in B-cells.
Clonal proliferation of B-cells usually results in a monoclonal
population expressing either kappa or lambda light chains.
The detection of clonal proliferation or messenger RNA (mRNA)
restriction for kappa or lambda light chains is commonly associated with
B-cell lymphomas and multiple myeloma. However, clonal proliferation
is not always diagnostic of malignancy.
Kappa and lambda light chains can be detected by in situ hybridization
assays using oligonucleotide probes in paraffin sections.
Reference Values
An interpretive report will be provided.
#5439 "Surgical Pathology Consultation" is required,
at an additional charge, along with this test.
Interpretation
A positive rection results in a blue staining in the cytoplasm in tissue
sections.
Cautions
Interpretation of this test should be performed in conjunction with
histologic examination of the hematoxylin-and-eosin stained sections
and other ancillary studies, such as immunohistochemistry.
Clinical Reference
1. Akhtar N, Ruprai A, Pringle JH, et al: In situ hybridization detection
of light chain mRNA in routine bone marrow trephines from patients
with suspected myeloma. Br J Haematol 1989;73:296-301
2. Weiss LM, Movahed LA, Chen YY, et al: Detection of immunoglobulin
light-chain mRNA in lymphoid tissues using a practical in-situ
hybridization method. Am J Pathol 1990;137:979-988
3. Ruprai AK, Pringle JH, Angel CA, et al: Localization of immunoglobulin
light-chain mRNA expression in Hodgkin's disease by in situ
hybridization. J Pathol 1991;164:37-40
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