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Diagnosis of Pneumocystis jiroveci pneumonia
When this test is ordered, the reflex test may be performed and charged.
Pneumocystis jiroveci is one of the major microbial pathogens associated with opportunistic pulmonary infections in patients receiving immunosuppressive therapy or with immune deficiencies. Presently, the most common means to diagnose Pneumocystis jiroveci infection is by microscopic detection of the organisms in specimens such as bronchoalveolar lavage, open lung biopsy tissue, induced sputum and transtracheal aspirate.
Negative: no cysts observed
Positive: cysts present
Only the cyst stage of Pneumocystis jiroveci stains by this method
1. Olsson M, Elvin K, Linder E, et al: Pneumocystis carinii is still a dangerous opportunist. The infection is continuously a threat to immunocompromised patients. Lakartidningen 1999 Jan27;96(4):328-331
2. Shelhamer JH, Gill VJ, Quinn TC, et al: The laboratory evaluation of opportunistic pulmonary infections. Ann Intern Med 1996 Mar 15;124(6):585-599
3. Kim YK, Parulekar S, Yu PK, et al: Evaluation of calcofluor white stain for detection of Pneumocystis carinii. Diagn Microbiol Infect Dis 1990;13:307-310
4. Baselski VS, Robison MK, Pifer LW, Woods DR: Rapid detection of Pneumocystis carinii in bronchoalveolar lavage samples by using Cellufluor staining. J Clin Microbiol 1990;28:393-394