Search our Test Catalog. Specify what to search (name, test code, titles, everywhere) and how to search (begins with, contains)

Unit Code 80027:
B-Cell Lymphoma, FISH, Tissue

Print Friendly View
Print Page Email Page

Useful For

Detecting chromosome anomalies in paraffin-embedded lymphoid

tissue

 

Providing diagnostic and prognostic genetic information for the following

lymphomas:  mantle cell, follicular, Burkitt, and diffuse large B-cell

lymphoma

 

We recommend this test in cases where the diagnosis is questionable,

or potential prognostic information may become available

Clinical Information

Lymphoid neoplasms are known to be complex, and the prognosis and

clinical course of patients with lymphoma is highly variable. Genetic

abnormalities have emerged as 1 of the most reliable criteria for

categorizing lymphomas. Several chromosome anomalies and variants

of these anomalies have been associated with various kinds of

lymphoma (see table below). Unfortunately, conventional chromosome

studies cannot be employed on paraffin-embedded tissue and molecular

genetic analyses are often problematic in the study of lymphomas. New

methods using chromosome-specific fluorescent-labeled DNA probes

and FISH now permit detection of abnormal genes associated with

various chromosome anomalies in lymphoma (see table below).

                                                                        

Common Chromosome Anomalies in Lymphomas

 

Lymphoma Type

Chromosome Anomaly

 

FISH Probe

Burkitt

t(8;14)(q24;q32)

c-MYC/IGH

Mantle Cell

t(11;14)(q13;q32)

CCND1/IGH

Follicular

t(14;18)(q32;q21)

IGH/BCL2

Diffuse large B-cell

t(3;var)(q27;var)

BCL6

t(14;18)(q32;q21)

IGH/BCL2

t(8;14)(q24;q32)

c-MYC/IGH

 

Reference Values

An interpretive report will be provided.

Interpretation

A neoplastic clone is detected when the percent of cells with an

abnormality exceeds the normal reference range for any given

probe.

Cautions

It is possible to order 1 or any combination of these probes, but

this must be done in consultation with 1 of our hematopathologists.

 

This test has not been approved by the Food and Drug Administration

(FDA) and is best used as an adjunct to existing clinical and pathological

information.

Special Instructions and Forms

• Informed Consent Form for DNA Testing 
• Cytogenetics Hematologic FISH Panel Patient Information Sheet 

Clinical Reference

1.   Jaffe ES: World Health Organization of Tumours. Pathology

      and Genetics of Tumours of Haematopoietic and Lymphoid Tissues.

      International Agency for Research on Cancer 2001

 

2.   Paternoster SF, Brockman SR, McCLure RF, et al:  A new method to

      extract nuclei from paraffin-embedded tissue to study lymphomas

      using interphase fluorescence in situ hybridization. Am J Pathol 2002;

      160(6):1967-1972

 

3.   Remstein ED, Kurtin PJ, Buno I, et al:  Diagnostic utility of

      fluorescence in situ hybridization in mantle-cell lymphoma. Br J

      Haematol 2000;110(4):856-862

 

4.   Remstein ED, Kurtin PJ, James CD, et al:  Mucosa-associated

      lymphoid tissue lymphomas with t(11;18)(q21;q21) and mucosa-

      associated lymphoid tissue lymphomas with aneuploidy develop

      along different pathogenetic pathways. Am J Pathol 2002;161:63-71

Key