Carboxy-Tetrahydrocannabinol (THC) Confirmation, Chain of Custody, Urine
Detection and confirmation of drug abuse involving delta-9-tetrahydrocannabinol (marijuana)
Chain of custody is required whenever the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was under the control of personnel involved with testing the specimen at all times; this control implies that the opportunity for specimen tampering would be limited.
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
Adulterants testing will be performed on all chain of custody urine samples as per regulatory requirements.
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Delta-9-tetrahydrocannabinol is the active agent of the popularly abused street drug, marijuana.
Following consumption of the drug, either by inhalation or ingestion, it is metabolized to a variety of inactive chemicals, 1 of them being delta-9-tetrahydrocannabinol carboxylic acid.
The immunochemical procedure used to screen for tetrahydrocannabinol (THC) as part of IDOAU / Drug Abuse Survey, Urine is designed to cross-react with THC carboxylic acid.
In almost all medico-legal cases and in screening of employees, or when the patient adamantly denies THC use and the immunochemical test is positive, confirmation of the result by gas chromatography-mass spectrometry and EIA are required.
Chain of custody is a record of the disposition of a specimen to document who collected it, who handled it, and who performed the analysis. When a specimen is submitted in this manner, analysis will be performed in such a way that it will withstand regular court scrutiny.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Positives are reported with a quantitative GC-MS result.
THC CARBOXYLIC ACID BY GC-MS
The presence of tetrahydrocannabinol carboxylic acid (THC-COOH), a major metabolite of delta-9-tetrahydrocannabinol, in urine at concentrations >15 ng/mL is a strong indicator that the patient has used marijuana.
The metabolite of marijuana (THC-COOH) has a long half-life and can be detected in urine for more than 7 days after a single use. The presence of THC-COOH in urine >100 ng/mL indicates relatively recent use, probably within the past 7 days. Levels >500 ng/mL suggest chronic and recent use. Chronic use causes accumulation of THC and THC-COOH in adipose tissue such that it is excreted into the urine for as long as 30 to 60 days from the time chronic use is halted.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
No significant cautionary statements
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Moyer TP, Palmen MA, Johnson P, et al: Marijuana testing-how good is it? Mayo Clin Proc 1987;62:413-417