Sex Chromosome Determination, FISH, Tissue
Identifying the sex chromosome complement in paraffin-embedded tissues
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Genotypically normal females possess 2 X chromosomes; genotypically normal males possess 1 X chromosome and 1 Y chromosome. Determining the sex chromosome complement in a tissue specimen can be used to:
-Identify opposite sex-donor cells post-transplant
-Help resolve cases of suspected sample mix-up
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
An interpretive report will be provided.
An interpretive report is provided.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
This test is not approved by the FDA and it is best used as an adjunct to existing clinical and pathologic information.
For testing of bone marrow samples from patients who have undergone an opposite sex transplant, order FBMT / XX/XY in Opposite Sex Bone Marrow Transplantation, FISH.
FISH analysis was performed on a series of patient specimens and results were compared to cytogenetic analyses. Using X and Y centromere probes, FISH analysis of interphase nuclei identified the correct chromosome complement. Specimens with known sex chromosome aneusomy were correctly identified.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Nakhleh RE, Zarbo RJ: Surgical pathology specimen identification and accessioning: A College of American Pathologists Q-Probes study of 1,004,115 cases from 417 institutions. Arch Pathol Lab Med 1996;120:227-233
2. Riopel MA, Yu IT, Hruban RH, et al: Whose tumor is this: FISHing for the answer. Mod Pathol 1995;8:456-457
3. Valenstein PN, Raab SS, Walsh MK: Identification Errors Involving Clinical Laboratories: A College of American Pathologists Q-Probes Study of Patient and Specimen Identification Errors at 120 Institutions. Arch Pathol Lab Med 2006;130:1106-1113