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Specimen Collection & Preparation

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Urine Specimens


Step 1:

Determine the Specimen Requirements

In the Test Catalog, use the Specimen and Overview tabs of each test to identify:

  • Patient preparation requirements
  • Specimen requirements
  • Specimen container requirements
  • Specimen stability (temperature) requirements
  • Collection instructions
  • Required forms or special instructions
  • If a preservative must be added at the start of the collection. See the
    Urine Preservatives Chart. This includes an additional chart for conditions and concentrations of urine preservatives.

Step 2:

Give Urine Collection Instructions to the Patient (24-hour Collections)

If a 24-hour collection is required, print the 24-Hour Urine Collection Instructions for the patient.

When you give the instructions and 24-hour collection container to the patient, review:

  • Collection duration
  • Diet requirements
  • Potentially hazardous preservatives in collection container
  • Storage of the specimen until it is returned

Step 3:

Pour the Specimen into an Aliquot Tube or Bottle

Mix well before aliquotting and provide total volume of random collection.

  • Send urine aliquots in the following leakproof containers only. Other containers could leak and compromise the specimen.
  • Some specimens require light protection to ensure specimen integrity. Check the Light Protection List before packaging your specimen, and use amber containers to prevent analyte degradation due to exposure to light (for 24-hour collections).
    Urine Tube
    Urine Container
    Amber Urine Container
T068 Urine Tube, 13mL


Standard aliquot tube for random collection.

T313 Urine Container, 60mL


Standard aliquot container for 24-hour collection.

T596 Urine Container, Amber, 60mL


Aliquot tube for 24-hour collections when testing for light-sensitive analytes.


Step 4:

Label the Specimen

Specimens must have two (2) person-specific identifiers on the patient label. Person-specific identifiers include:

  • Accession number
  • Patient's first and last name
  • Unique identifying number (eg, medical record number)
  • Date of birth

Mislabeled Specimens

Specimens are considered mislabeled when there is a mismatch between the person-specific identifiers on the specimen and the information accompanying the specimen. This information might include a computer system, requisition form, or additional paperwork.

In addition, if there is a handwritten name on the container and a label on the container, the information must match exactly. For example, "Rebecca" does not match "Becky." When insufficient or inconsistent identification is submitted, a new specimen may be required.


Step 5:

Package the Specimen in a Biohazard Bag

Biohazard Shipping Bags

T229 Refrigerate Bag - pink
T027 Ambient Bag - white
T121 Frozen Bag - yellow


Place the tube or container in a Mayo Medical Laboratories color-coded (temperature-specific) shipping bag.












12x15 Biohazard Bag

T043 Biohazard Bag - 12x15              
Use if your container is too
large for color-coded bags.
Mark it Frozen, Refrigerate
or Room Temp (Ambient).


Electronic Clients:

  • Clients who submit electronic orders will have a batch order. Place all specimens for the temperature-specific batch number into one bag. If all the specimens do not fit, use a larger biohazard bag (T043) and indicate the shipping temperature.
  • Do not place multiple batches into one bag.







If you are not using a bag supplied by Mayo Medical Laboratories:
  • The bag must be leakproof.
  • There must be absorbent material between the primary receptacle (tube/container) and the secondary packaging (bag) that is able to absorb the entire contents of the bag.
  • Wrap any breakable tubes individually in bubble wrap (T055).

Step 6:

Package Batch Sheets and Forms

Folded batch sheet in outside pocket of bag

Folded batch sheet with
bar code and delivery
address visible

Electronic Clients

Clients who submit electronic orders will have a batch sheet. The bottom of the batch sheet lists the number of pages (eg, 1 of 3). Fold the batch sheets in fourths and place them in the outside pocket of the bag. If there is no pocket, place them inside the bag with the specimens. Include all pages in the corresponding bag. Delivery address and bar code if applicable must be visible. Do not combine multiple batches into 1 bag.

Manual Clients

Clients who do not order electronically must include a completed Test Request form with each patient specimen. Our Customized Test Request Form generator provides the Test Request form and all other required forms for the specified test. Complete and print all forms included, then fold and insert the forms into the outside pocket of the biohazard bag. If there is no pocket, place them inside the bag with the specimen.



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