Interpretive Handbook

Test 80172 :
Human Papillomavirus (HPV) Typing, DNA In Situ Hybridization

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Human papillomavirus (HPV) infections with low-risk genotypes can cause benign hyperplasia such as condylomas and papillomas. Persistent infections with high-risk HPV are associated with cervical, vaginal, vulvar, and head and neck malignancies. Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC.

Useful For Suggests clinical disorders or settings where the test may be helpful

Detection of both low-risk and high-risk genotypes of human papillomavirus DNA in paraffin-embedded human tissue

Interpretation Provides information to assist in interpretation of the test results

This test will be processed as a special procedure.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

The probe set used in this human papillomavirus (HPV) DNA in situ hybridization (ISH) test cannot detect all potential HPV serotypes that are associated with oropharyngeal squamous cell carcinoma. Following a negative DNA ISH result, a more sensitive in situ RNA test could be performed, if clinically indicated. The RNA test is only available in the context of a pathology consultation (70012 / Pathology Consultation).

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Results are reported as positive or negative for low-risk genotypes 6, 11, and/or high-risk genotypes 16, 18, 31, 33, and 51.

If additional interpretation/analysis is needed, please request 70012 / Pathology Consultation along with this test.

Clinical References Provides recommendations for further in-depth reading of a clinical nature

1. Kelesidis T, Aish L, Steller MA, et al: Human papillomavirus (HPV) detection using in situ hybridization in histologic samples. Am J Clin Pathol 2011;136:119-127

2. Lee WT, Tubbs RR, Teker AM, et al: Use of in situ hybridization to detect human papillomavirus in head and neck squamous cell carcinoma patients without a history of alcohol or tobacco use. Arch Pathol Lab Med 2008;132:1653-1656

3. Birner P, Bachtiary B, Dreier B, et al: Signal-amplified colorimetric in situ hybridization for assessment of human papillomavirus infection in cervical lesions. Mod Pathol 2001;14(7):702-709