|Values are valid only on day of printing.|
The presence of bilirubin in amniotic fluid, which results in a yellow color, is an indicator of fetal erythroblastosis. Visual inspection of amniotic fluid is unreliable because bilirubin is not the only cause of an excessive yellow color; therefore, the presence of bilirubin must be confirmed with spectrophotometric methods. Meconium may contribute a green color (biliverdin) that can obscure the color of bilirubin and hemoglobin.
Evaluation of Rh disease, ie, hemolytic disease of the fetus
Monitoring disease progression to assess need for fetal transfusion
The reference range for bilirubin in amniotic fluid is related to the gestational age of the fetus. Refer to either the Queenan Curve (gestational age <27 weeks) or the Liley Chart (gestational age >27 weeks) listed under Interpretation of Amniotic Fluid Bilirubin Results (Delta OD 450) in Special Instructions.
Bilirubin is photosensitive. Collect the specimen in an opaque amber container and/or wrap in aluminum foil.
Interpretation of fetal risk is dependent upon gestational age.
Refer to either the Queenan Curve (gestational age <27 weeks) or the Liley Chart (gestational age >27 weeks) listed under Interpretation of Amniotic Fluid Bilirubin Results (Delta OD 450) in Special Instructions.
1. Scott F, Chan FY: Assessment of the clinical usefulness of the 'Queenan' chart versus the 'Liley' chart in predicting severity of rhesus iso-immunization. Prenat Diagn 1998;18:1143-1148
2. Liley AW: Liquor amnii analysis in the management of the pregnancy complicated by rhesus sensitization. Am J Obstet Gynecol 1961;82:1359-1370