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Despite advances in water treatment, food safety, and sanitary conditions, acute diarrheal disease remains a leading cause of morbidity and mortality worldwide. Most bacterial enteric infections in the United States originate within the food supply chain. According to the CDC, 43% of laboratory-confirmed bacterial enteric infections in the United States are caused by Salmonella species, followed by Campylobacter species (33%), Shigella species (17%), Shiga toxin-producing Escherichia coli (4.1%), and Yersinia species (0.9%).(1)
Although Salmonella, Campylobacter, Shigella, and Yersinia species, and Escherichia coli O157:H7 are easily cultivated on standard selective and differential bacteriologic media, isolation and final identification are time consuming, delaying diagnosis for several days and putting patients at risk for untreated infection and spread of infection to others. For example, the time to final identification from stool culture for Salmonella, Shigella, and Yersinia species ranges from 3 to 5 days, and for Campylobacter species it ranges from 2 to 4 days. Furthermore, non-O157:H7 Shiga toxin-producing Escherichia coli are not readily isolated in the clinical laboratory.
PCR detection of the most common agents of bacterial enteric infections directly from stool specimens is sensitive, specific, and provides same-day results, eliminating the need for culture in most cases. In addition, PCR provides physicians with a new tool in their care of patients and public health personnel in their investigation and control of the spread of these diseases.
Rapid, sensitive, and specific identification of the common bacterial causes of diarrhea; Campylobacter jejuni/coli, Salmonella species, Shigella species, enteroinvasive Escherichia coli, Shiga toxin-producing Escherichia coli, and Yersinia species
A positive PCR result for any 1 of the specific assays (Campylobacter jejuni/coli, Salmonella species, Shigella species/enteroinvasive Escherichia coli, Shiga toxin-producing Escherichia coli, or Yersinia species) indicates the presence of the respective organism in the specimen.
A negative result indicates the absence of detectable Salmonella species, Shigella species/enteroinvasive Escherichia coli, pathogenic Yersinia species, Campylobacter jejuni/coli, and shiga toxin DNA in the specimen, but does not rule-out infection with these or other enteric pathogens. False-negative results may occur due to inhibition of PCR (known inhibition rate of <1%), sequence variability underlying the primers and probes or the presence of enteric pathogens in quantities less than the limit of detection of the assay or not targeted by the assays (eg, Vibrio cholerae).
Interfering substances in stool may affect the accuracy of this assay; results should always be interpreted in conjunction with clinical and epidemiological findings.
This test does not detect Aeromonas species, Vibrio species, Plesiomonas shigelloides, enterotoxigenic Escherichia coli, or other pathogens not specifically listed.
1. Centers for Disease Control and Prevention. Preliminary FoodNet Data on the incidence of infection with pathogens transmitted commonly through food--10 States, 2008. MMWR Morb Mortal Wkly Rep 2009;58(13):333-337
2. Cunningham SA, Sloan LM, Nyre LM, et al: Three-hour molecular detection of Campylobacter, Salmonella, Yersinia, and Shigella species in feces with accuracy as high as that of culture. J Clin Microbiol 2010;48:2929-2933
3. Grys TE, Sloan LM, Rosenblatt JE, Patel R: Rapid and sensitive detection of Shiga toxin-producing Escherichia coli from nonenriched stool specimens by real-time PCR in comparison to enzyme immunoassay and culture. J Clin Microbiol 2009;47(7):2008-2012