|Values are valid only on day of printing.|
Nitrogen is a key component of proteins. Nitrogen balance is the difference between the amount of nitrogen ingested and the amount excreted in the urine and feces. A majority of nitrogen is excreted as urea in the urine, however, fecal nitrogen can account for 30% to 50% of total nitrogen excretion.
A patient who is in negative nitrogen balance is catabolizing muscle protein to meet the metabolic requirements of the protein catabolism and,therefore, urine and fecal nitrogen may be increased due to stress, physical trauma, surgery, infections, burns, and 11-oxysteroid or thyroxine use. Testosterone and growth hormone have anabolic effects on protein synthesis and may decrease urine and fecal nitrogen.
In the course of chronic progressive pancreatitis, as the pancreas is destroyed, serum amylase and lipase may revert to normal. However, excessive fecal nitrogen levels persist and are used as an indicator of pancreatic atrophy.
Determining nitrogen balance, when used in conjunction with 24-hour urine nitrogen measurement
Assessing nutritional status (protein malnutrition)
Evaluating protein catabolism
Average fecal nitrogen excretion is approximately 1-2 g N/24 hours. Significantly abnormal excretion rates, resulting in negative nitrogen balance, may be associated with severe stress due to multiple trauma, head injury, sepsis, or extensive burns. Elevated values >2.5 g N/24 hours may be consistent with chronic progressive pancreatitis. The goal with therapy for a depleted person is a positive nitrogen balance of 4 to 6 g N/24 hours.
Send ENTIRE STOOL COLLECTION (must contain at least 5 g of feces) frozen on dry ice in Mayo Medical Laboratories approved container.
Measurement of both urine and fecal nitrogen is necessary for the accurate determination of nitrogen balance.
Specify length of collection period (duration of either 24, 48, 72, or 96 hour) for entire stool specimen.
During nitrogen balance studies, nitrogen lost from exuding wounds, such as burns, and from copious sputum must be included in the patient's evaluation.
<16 years: not established
> or =16 years: 1-2 g/24 hours
1. Morse, MH, et al: Protein requirement of elderly women: nitrogen balance responses to three levels of protein intake. J Gerontol A Biol Sci Med Sci. 2001 Nov;56(11):M724-730
2. Phinney SD: The assessment of protein nutrition in the hospitalized patient. Clin Lab Med 1981;1:767-774
3. Konstantinides FN, Kostantinides NN, Li JC, et al: Urinary urea nitrogen: too insensitive for calculating nitrogen balance studies in surgical clinical nutrition. J Parenter Enteral Nutr 1991;15:189-193