Leukemia/Lymphoma Immunophenotyping by Flow Cytometry, Tissue
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Cellular immunophenotyping, characterizing cells by using antibodies directed against cell surface markers, is generally regarded as a fundamental element in establishing a diagnosis of tissue involvement by hematolymphoid malignancies, when used in conjunction with morphologic assessment. It is also an essential component in subclassification of hematolymphoid malignancies, when present.
Evaluation of tissues for potential involvement by:
-Chronic lymphoproliferative disorders
-Acute lymphoblastic leukemia
-Acute myelogenous leukemia
Normal tissues typically contain a mixture of B cells with polytypic surface immunoglobulin light chain expression and T cells with unremarkable expression of the T cell-associated antigens CD3, CD5, and CD7. Typically, no appreciable blast population is present by CD45 and side scatter analysis.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
It is well recognized that a negative flow cytometry result does not exclude tissue involvement by hematolymphoid malignancy. This may be attributable to sampling bias, although some malignancies, such as Hodgkin lymphoma, are not detected by this technique.
Viability will be assessed in all tissue specimens. Cases in which the viability is low (<50%) are prone to false-negative results and, therefore, must be interpreted with caution. In cases with viability <30%, testing will be attempted but may not be interpretable. Fine-needle aspiration and small biopsy specimens have a higher frequency of low cell counts and/or poor viability which may be uninterpretable.
Even when abnormal, in most instances the results of flow cytometry are insufficient for complete subclassification of a hematolymphoid malignancy. Precise subclassification requires correlation with the histopathologic features in paraffin-embedded materials and also, in some instances, the results of cytogenetic analyses.
The tissue used for flow cytometry cannot be subsequently submitted for histopathologic evaluation. For this reason, this technique should be avoided in small biopsy specimens.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
An interpretive report will be provided.
Clinical References Provides recommendations for further in-depth reading of a clinical nature
1. Morice WG, Hodnefield JM, Kurtin PJ, Hanson CA: An unusual case of leukemic mantle cell lymphoma with a blastoid component showing loss of CD5 and aberrant expression of CD10. Am J Clin Pathol 2004;July;122(1):122-127
2. Hanson CA: Acute Leukemias and Myelodysplastic Syndromes. In Clinical Laboratory Medicine. Edited by KD McClatchey. Baltimore, MD, Williams and Wilkins, 1994, pp 939-969
3. Jaffe ES, Cossman J: Immunodiagnosis of Lymphoid and Mononuclear Phagocytic Neoplasms. In Manual of Clinical Immunology. Third Edition. Edited by NR Rose, H Friedman, JL Fahey. American Society for Microbiology, 1987, pp 779-790
4. Witzig TE, Banks PM, Stenson MJ, et al: Rapid immunotyping of B-cell non-Hodgkin’s lymphomas by flow cytometry. Am J Clin Pathol 1989;94:280-286
5. Flow Cytometry in Clinical Diagnosis, Fourth Edition. Edited by P Keren, JP McCoy Jr, J Carey J. Chicago, IL, ASCP Press, 2007