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Interpretive Handbook

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Test 89185:
HLA Class I Molecular Typing Disease Association

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Human leukocyte antigens (HLA) are regulators of the immune response that play a key role in transplantation. HLA class I typing is most frequently applicable to organ transplant donor/recipient matching, provision of HLA-matched platelets for alloimmunized refractory patients, and for a small number of disease associations.

 

Class I HLA antigens include A, B, and C loci. This assay is designed to provide low-to-medium resolution for HLA class I typing (A, B, C). Low-to-medium resolution defines the typing at the antigen level. This is in contrast to high-resolution typing, which defines typing at the allele (molecular) level and is used primarily for typing donor/recipient pairs for unrelated bone marrow transplantation.

Useful For Suggests clinical disorders or settings where the test may be helpful

Determining class I HLA antigens on specimens for transplant candidates and their donors or those who have become refractory to platelet transfusions

Interpretation Provides information to assist in interpretation of the test results

Interpretation depends on the rationale for ordering the test. Assessments of acceptable donor/recipient matches are made on a case-by-case basis.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

No significant cautionary statements

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Not applicable

Clinical References Provides recommendations for further in-depth reading of a clinical nature

1. Terasaki PI, Bernoco D, Park MS, et al: Microdroplet testing for HLA-A, B, C and D antigens. Am J Clin Pathol 1978 Feb;69(2):103-120

2. Colinas RJ, Bellisario R, Pass KA: Multiplexed genotyping of beta-globin variants form PCR-amplified newborn blood spot DNA by hybridization with allele-specific oligodeoxynucleotides coupled to an array of fluorescent microspheres. Clin Chem 2000 Jul;46(7):996-998