Interpretive Handbook

Test 60483 :
Human Papillomavirus (HPV), High-Risk, DNA In Situ Hybridization

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Persistent infections with high risk human papillomavirus (HPV) genotypes (16, 18, 31, 33 and 51) are associated with cervical, vaginal, vulvar, and head and neck malignancies. Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC.

Useful For Suggests clinical disorders or settings where the test may be helpful

Detection of human papillomavirus DNA from high-risk genotypes (16, 18, 31, 33 and 51)

Interpretation Provides information to assist in interpretation of the test results

This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation/analysis is needed, please request test 70012 / Pathology Consultation along with this test.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

The probe set used in this human papillomavirus (HPV) DNA in situ hybridization (ISH) test cannot detect all potential HPV serotypes that are associated with oropharyngeal squamous cell carcinoma. Following a negative HPV High Risk DNA ISH result, a more sensitive test, High Risk HPV RNA ISH, could be performed, if clinically indicated. The RNA ISH test is only available in the context of a pathology consultation (test 70012 / Pathology Consultation).

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.


Clinical References Provides recommendations for further in-depth reading of a clinical nature

1. Kelesidis T, Aish L, Steller MA, et al: Human papillomavirus (HPV) detection using in situ hybridization in histologic samples. Am J Clin Pathol 2011;136:119-127

2. Lee WT, Tubbs RR, Teker AM, et al: Use of in situ hybridization to detect human papillomavirus in head and neck squamous cell carcinoma patients without a history of alcohol or tobacco use. Arch Pathol Lab Med 2008;132:1653-1656

3. Birner P, Bachtiary B, Dreier B, et al: Signal-amplified colorimetric in situ hybridization for assessment of human papillomavirus infection in cervical lesions. Mod Pathol 2001;14(7):702-709