Bacterial Culture, Aerobic, Urine
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Urinary tract infection (UTI) encompasses a broad range of clinical entities that vary in their clinical presentation, degree of tissue invasion, epidemiologic setting, and antibiotic therapy requirements. There are four major types of UTIs: urethritis, cystitis, acute urethral syndrome, and pyelonephritis. UTIs may also be classified as uncomplicated or complicated. Escherichia coli is the leading cause of uncomplicated community-acquired UTI. Risk factors that predispose one to complicated UTIs include: underlying diseases that are associated with kidney infection (eg, diabetes), kidney stones, structural or functional urinary tract abnormalities and indwelling urinary catheters. Another classification of UTIs is as upper UTI (related to the kidney, renal pelvis, or ureter) or lower UTI (urinary bladder and urethra). The classic symptoms of upper UTI are fever (often with chills) and flank pain; frequent painful urination, urgency, and dysuria are more often associated with lower UTI.
Diagnosis of urinary tract infections
Quantitative culture results may be helpful in discriminating contamination, colonization, and infection.
In general, the isolation of >100,000 cfu/mL of a urinary pathogen is indicative of urinary tract infection. Isolation of 2 or more organisms >10,000 cfu/mL may suggest specimen contamination.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Although urine is normally sterile, contamination by organisms normally present in the urethra or on periurethral surfaces can allow a proliferation of these organisms yielding misleading urine culture results.
Urine held at room temperature for >30 minutes supports the growth of both pathogens and contaminants, leading to potentially inaccurate colony counts.
Urine obtained from catheter bags at the bedside and Foley catheter tips are unacceptable for culture.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Identification of probable pathogens with colony count ranges
Clinical References Provides recommendations for further in-depth reading of a clinical nature
Mandell GL, Bennett JE, Dolin R: Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. Fourth edition. New York, Churchill Livingstone, 1995, pp 662-690