Real-Time PCR for the Clinical Microbiology Laboratory

Real-Time PCR for the Clinical Microbiology Laboratory

November 15-16, 2007

Upcoming Events

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Laboratory Outreach Sales Fundamentals and Best Practices Reinforcement – Part Three of Three
November 18, 2008

Outreach Forum Conference:
Your Outreach Market: Understanding and Projecting Opportunity
November 20, 2008

Disease Management Strategies
Troponin and BNP: The More We Learn, the More Questions We Have
December 02, 2008

Quality Management Series
Driving Value and Quality into the Laboratory
December 08, 2008

Contact Information

Mayo Medical Laboratories
Education Department

E-Mail Address
Phone: 800.533.1710
Fax: 507.284.8016
Global: 507.284.3156

Real-Time PCR for the Clinical Microbiology Laboratory

Conference Agenda

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Thursday, November 15, 2007

7:00 AM Registration and Continental Breakfast
Phillips Hall Foyer, First Floor
Harold W. Siebens Medical Education Building
7:30 AM Orientation and Introduction
Franklin R. Cockerill III, MD
7:45 AM Overview of Rapid Cycle Real-Time PCR Technology
Franklin R. Cockerill III, MD
8:30 AM Specimen Processing
Jon E. Rosenblatt, MD
8:45 AM Nucleic Acid Extraction
Mark J. Espy, MS
9:00 AM Panel Discussion
9:15 AM Break
Phillips Hall Foyer, First Floor
Harold W. Siebens Medical Education Building
9:45 AM GeneXpert Extraction/Detection System
Scott A. Cunningham, MT (ASCP) SM
10:00 AM Real-Time PCR Detection Chemistries
Seanne P. Buckwalter, MS
10:20 AM Gene Target Selection and Assay Optimization of Real-Time PCR Assays
Lynne M. Sloan, MT (ASCP)
10:40 AM Training and Workflow Strategies for Real-Time PCR Assays
Scott A. Cunningham, MT (ASCP) SM
11:00 AM Panel Discussion
11:15 AM Real Time PCR Assays in the Virology Laboratory
Thomas F. Smith, PhD
11:45 AM Viral ASR’s for the SmartCycler
Mark J. Espy, MS
12:00 AM Panel Discussion
12:15 PM Lunch
Rochester Marriott Hotel
Ballroom, Second Floor
1:15 PM Applications for Bacterial Diagnostics
Franklin R. Cockerill III, MD
1:45 PM Using a Patient Focused Approach for Implementation of Real-Time Assays
Emily A. Vetter, MT (ASCP)
2:00 PM Panel Discussion
2:15 PM Break
Phillips Hall Foyer, First Floor
Harold W. Siebens Medical Education Building
2:45 PM Quality Control and Managing Contamination Events with Real-Time Technologies
Seanne P. Buckwalter, MS
3:10 PM Verification of Real-Time Assays: Recommendations and Requirements
Lynne M. Sloan, MT(ASCP)
3:45 PM Panel Discussion
6:30 PM Conference Reception
Rochester Marriott Hotel
Ballroom, Second Floor

Friday, November 16, 2007

7:00 AM Continental Breakfast
Phillips Hall Foyer, First Floor
Harold W. Siebens Medical Education Building
8:00 AM Virtual Laboratory Tour
8:30 AM Extraction Methods
• Swab extraction/lysis
9:00 AM Break
Phillips Hall Foyer, First Floor
Harold W. Siebens Medical Education Building
9:15 AM Nucleic Acid Extraction
• Manual extraction kits
• Automated extractors
• Extraction control recommendations
11:30 AM Lunch
Rochester Marriott Hotel
Ballroom, Second Floor
12:30 PM Operation of the LightCyler and SmartCycler

FDA Approved and ASR Real-Time PCR assays
• Single assays
• Multiplex assays
• Quantitative assays
• Weak positive results and repeat testing
2:30 PM Adjourn

 

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