Coagulation Wet Workshop

 

Workshop Overview

Tuesday, August 3, 2010

Following orientation presentations, participants will form teams for testing and evaluation of four “unknown” plasma specimens using coagulation instrumentation, reagents and technical specialists at each of five planned workstations.

Testing will include prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), dilute Russell’s viper venom time (DRVVT), mixing tests using unknown and normal plasmas, platelet neutralization procedure (PNP) and other phospholipid-inhibitor confirmatory assays. Additional testing will include assays of fibrinogen and selected coagulation factor activities (factors II, V, VII, VIII).

Workshop faculty will lead a discussion of results and interpretations, including comments about the clinical disorders studied and the comparability or diversity of results and interpretations obtained with different instruments or reagents. Participation is limited to 100 registrants.

Objectives

Upon completion of this workshop, participants should be able to:

• Examine operation of contemporary coagulation instrumentation and reagents
• Discern similarities and differences among coagulation reagents and instruments
• Assess performance characteristics of different reagents and instruments for evaluating relatively common complex coagulopathies
• Evaluate “screening tests” and “confirmatory tests” for detection, differentiation, and confirmation of differing coagulopathies
• Explore “mixing tests” for differentiating inhibitors of coagulation from coagulation factor deficiencies
• Discuss principles and performance of coagulation factor activity assays
• Analyze the diagnostic sensitivity and specificity of results obtained with differing reagent instrument combinations and test procedures.

Intended Audience

This workshop is designed for laboratory technologists, physicians, and scientists who have responsibilities for diagnostic coagulation laboratory testing.

Workstations

Workstations can take a maximum of 20 participants per session. Four assignments will be allotted per attendee. Below is a list of companies and coagulation instruments that will make up each of the workstations.

Beckman Coulter, Inc. - ACL TOP
Beckman Coulter, Inc. - ACL TOP 500 CTS
Diagnostica Stago - STAR Evolution
Siemens Healthcare Diagnostics - BCS XP
Trinity Biotech - Destiny MAX