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Anaerobic Bacteriology


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AST Methods

Slide 24

June 2012

Methods for anaerobic susceptibility testing include the CLSI reference agar dilution method which is primarily used as a standard for comparison of other methods.  However, it is generally not suitable for clinical laboratory use.  Broth micro dilution panel plates are commercially available.  They are somewhat time consuming and cumbersome and one is restricted to use of the antibiotics which are included in the panel on that particular plate.  The E-test method is a concentration gradient strip agar diffusion method which is probably most practical for the average clinical laboratory.  There is also a commercially available method called the MIC evaluator.  However, currently, it is available only for purchase in Europe.  The E-test method requires forty eight hours of incubation and will provide both an MIC and categorical value result.

AST Methods


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