Anaerobic Bacteriology

Antimicrobial Susceptibility Testing

June 2012

Antimicrobial susceptibility testing of anaerobic bacteria can be utilized for individual patient care on isolates from blood, the brain, bone and joints, when there is critical or long-term treatment considerations or when there is an unpredictable antimicrobial susceptibility pattern anticipated for the isolated organism.  It can also be employed when there has been a treatment failure.  Another option for performing susceptibility testing is to establish a hospital antibiogram by testing annually batches of organisms and posting of the results to serve as a guide for local physicians and pharmacists.

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Antimicrobial Susceptibility Testing

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Jump to section:

• Introduction
• Objectives
• Anaerobic Bacteria
• Principal Anaerobic Pathogens
• Specimen Selection: Avoid Contamination With Normal Flora
• Inappropriate Specimens
• Collection and Transport
• Anaerobic Transport Vials
• Anaerobic Transporters
• Anaerobic Culture Media
• Primary Culture CO₂ Holding Jar with Flow Meter
• Incubation
• Anaerobic Jars Set Up Using the Anaero-Pack
• Anaerobic Glove Box
• Anaerobe Culture Triage
• Anaerobe Culture Triage (cont.)
• Identification
• Colonial Morphology
• Pigmented Colonies
• Rapid Identification Using Gram Stain
• Identification
• 16S Ribosomal RNA Sequence of Bacteroides fragilis
• Antimicrobial Susceptibility Testing
• AST Methods
• Illustration of the Components of the E-test Method
• Susceptibility Testing by E-test
• Anaerobe Antimicrobial Panels
• References
• Special Thanks
• Questions