Anaerobic Bacteriology

Identification

June 2012

Identification initially is determined on the basis of growth on selective or differential media, the gram stain and colonial morphology, present of pigmentation on colonies, hydrolysis of esculin and fluorescence under UV light.  Rapid test which can be initially helpful are zones of inhibition around Kanamycin, Vancomycin and colistin disks or rapid indole, urease, nitrate and catalase test.  We also employ a beta-lactamase or Cefinase disk on gram-negative anaerobic bacteria.  Egg yolk agar may also be utilized to determine the production of lecithinase and lipase.

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Identification

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• Introduction
• Objectives
• Anaerobic Bacteria
• Principal Anaerobic Pathogens
• Specimen Selection: Avoid Contamination With Normal Flora
• Inappropriate Specimens
• Collection and Transport
• Anaerobic Transport Vials
• Anaerobic Transporters
• Anaerobic Culture Media
• Primary Culture CO₂ Holding Jar with Flow Meter
• Incubation
• Anaerobic Jars Set Up Using the Anaero-Pack
• Anaerobic Glove Box
• Anaerobe Culture Triage
• Anaerobe Culture Triage (cont.)
• Identification
• Colonial Morphology
• Pigmented Colonies
• Rapid Identification Using Gram Stain
• Identification
• 16S Ribosomal RNA Sequence of Bacteroides fragilis
• Antimicrobial Susceptibility Testing
• AST Methods
• Illustration of the Components of the E-test Method
• Susceptibility Testing by E-test
• Anaerobe Antimicrobial Panels
• References
• Special Thanks
• Questions