Anaerobic Bacteriology

Incubation

June 2012

Incubation is carried out in GasPak jars that are either mechanically flushed with an anaerobic gas or made anaerobic through the use of commercial products such as Anaero-Packs or GasPaks.  All jars should have a methylene blue EH indicator to ensure that anaerobiosis has been accomplished.  One may also use an anaerobic glove box either for primary incubation or for examination and work on plates which have been set up in anaerobic jars.

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Incubation

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Jump to section:

• Introduction
• Objectives
• Anaerobic Bacteria
• Principal Anaerobic Pathogens
• Specimen Selection: Avoid Contamination With Normal Flora
• Inappropriate Specimens
• Collection and Transport
• Anaerobic Transport Vials
• Anaerobic Transporters
• Anaerobic Culture Media
• Primary Culture CO₂ Holding Jar with Flow Meter
• Incubation
• Anaerobic Jars Set Up Using the Anaero-Pack
• Anaerobic Glove Box
• Anaerobe Culture Triage
• Anaerobe Culture Triage (cont.)
• Identification
• Colonial Morphology
• Pigmented Colonies
• Rapid Identification Using Gram Stain
• Identification
• 16S Ribosomal RNA Sequence of Bacteroides fragilis
• Antimicrobial Susceptibility Testing
• AST Methods
• Illustration of the Components of the E-test Method
• Susceptibility Testing by E-test
• Anaerobe Antimicrobial Panels
• References
• Special Thanks
• Questions