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Anaerobic Bacteriology



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Anaerobic Culture Media

Slide 10

June 2012

Once the specimen has reached the laboratory, it should be inoculated on fresh enriched, selective, and differential agar plates.  Examples are Brucella or CDC Blood Agar, Laked Blood Agar containing Kanamycin and Vancomycin or LKV, bile-esculin agar, phenylethyl alcohol agar or PEA, and thioglycollate broth tubes.  So called PRAS media that is pre-reduced anaerobically sterilized may be utilized but it is quite expensive and cumbersome to use and generally not necessary for the clinical laboratory.  Inoculated plates are then placed in a holding jar until that jar is full and ready to be set up as an anaerobic jar or for use in an anaerobic chamber glove box.  Initial incubation is for forty eight hours before the plates undergo examination.  Plates should also be checked for new growth for up to seven days and thioglycolate broth tubes should be kept for a total of fourteen days especially if one suspects infection of the total joint arthroplasty.

Anaerobic Culture Media

 


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