Anaerobic Bacteriology

Anaerobic Transport Vials

June 2012

This slide is an illustration of the anaerobic transport vials which are in use in the microbiology laboratory at the Mayo Clinic.  On the left is a sterile carbon dioxide filled tube for use with tissues.  After the tissue is placed in the tube the cap can be resealed.  On the right, is an illustration of an anaerobic vial containing peptone yeast extract with resazurin indicator to insure that not excessive oxygen has been introduced.

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Anaerobic Transport Vials

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Jump to section:

• Introduction
• Objectives
• Anaerobic Bacteria
• Principal Anaerobic Pathogens
• Specimen Selection: Avoid Contamination With Normal Flora
• Inappropriate Specimens
• Collection and Transport
• Anaerobic Transport Vials
• Anaerobic Transporters
• Anaerobic Culture Media
• Primary Culture CO₂ Holding Jar with Flow Meter
• Incubation
• Anaerobic Jars Set Up Using the Anaero-Pack
• Anaerobic Glove Box
• Anaerobe Culture Triage
• Anaerobe Culture Triage (cont.)
• Identification
• Colonial Morphology
• Pigmented Colonies
• Rapid Identification Using Gram Stain
• Identification
• 16S Ribosomal RNA Sequence of Bacteroides fragilis
• Antimicrobial Susceptibility Testing
• AST Methods
• Illustration of the Components of the E-test Method
• Susceptibility Testing by E-test
• Anaerobe Antimicrobial Panels
• References
• Special Thanks
• Questions