Anaerobic Bacteriology

Collection and Transport

June 2012

Collection and transport can be accomplished using needle and syringe aspirates of fluid or pus or from surgically obtained tissue.  Again, avoid the use of swabs whenever possible.  Fluid or pus is injected into sealed anaerobic transport vials and these should contain an EH indicator in the fluid or gel or agar in the bottom of the tube.  Place tissues in upright oxygen-free tubes which contain an anaerobic gas or carbon dioxide and seal the tube.  It is optimal to culture the specimen within two to three hours of collection.

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Jump to section:

• Introduction
• Objectives
• Anaerobic Bacteria
• Principal Anaerobic Pathogens
• Specimen Selection: Avoid Contamination With Normal Flora
• Inappropriate Specimens
• Collection and Transport
• Anaerobic Transport Vials
• Anaerobic Transporters
• Anaerobic Culture Media
• Primary Culture CO₂ Holding Jar with Flow Meter
• Incubation
• Anaerobic Jars Set Up Using the Anaero-Pack
• Anaerobic Glove Box
• Anaerobe Culture Triage
• Anaerobe Culture Triage (cont.)
• Identification
• Colonial Morphology
• Pigmented Colonies
• Rapid Identification Using Gram Stain
• Identification
• 16S Ribosomal RNA Sequence of Bacteroides fragilis
• Antimicrobial Susceptibility Testing
• AST Methods
• Illustration of the Components of the E-test Method
• Susceptibility Testing by E-test
• Anaerobe Antimicrobial Panels
• References
• Special Thanks
• Questions