Anaerobic Bacteriology

Inappropriate Specimens

June 2012

Inappropriate specimens for anaerobic culture include Sputum, BAL’s, nasopharyngeal or oropharyngeal fluids.  Draining sinuses to the skin except when actinomycosis is suspected.  Open wounds, voided urine, cervical and vaginal samples, and any specimen which is grossly contaminated by feces including peritoneal fluid and there has been perforation of the intestinal mucosa.  The only exception for culture feces is when we are trying to diagnose Clostridium difficile infection.  This will not be further discussed during this session.

< Previous Slide

Inappropriate Specimens

Next Slide >

 

Jump to section:

• Introduction
• Objectives
• Anaerobic Bacteria
• Principal Anaerobic Pathogens
• Specimen Selection: Avoid Contamination With Normal Flora
• Inappropriate Specimens
• Collection and Transport
• Anaerobic Transport Vials
• Anaerobic Transporters
• Anaerobic Culture Media
• Primary Culture CO₂ Holding Jar with Flow Meter
• Incubation
• Anaerobic Jars Set Up Using the Anaero-Pack
• Anaerobic Glove Box
• Anaerobe Culture Triage
• Anaerobe Culture Triage (cont.)
• Identification
• Colonial Morphology
• Pigmented Colonies
• Rapid Identification Using Gram Stain
• Identification
• 16S Ribosomal RNA Sequence of Bacteroides fragilis
• Antimicrobial Susceptibility Testing
• AST Methods
• Illustration of the Components of the E-test Method
• Susceptibility Testing by E-test
• Anaerobe Antimicrobial Panels
• References
• Special Thanks
• Questions