Anaerobic Bacteriology

Principal Anaerobic Pathogens

June 2012

Principal anaerobic pathogens include the Bacteroides fragilis group, Fusobacterium, Prevotella and Porphyromonas, Bilophila, the anaerobic cocci, Clostridium, Propionibacterium and other nonspore forming gram-positive bacteria.  It’s important to recognize that there have been much recent change in the taxonomy of anaerobes due to development of new molecular methods.

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Principal Anaerobic Pathogens

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Jump to section:

• Introduction
• Objectives
• Anaerobic Bacteria
• Principal Anaerobic Pathogens
• Specimen Selection: Avoid Contamination With Normal Flora
• Inappropriate Specimens
• Collection and Transport
• Anaerobic Transport Vials
• Anaerobic Transporters
• Anaerobic Culture Media
• Primary Culture CO₂ Holding Jar with Flow Meter
• Incubation
• Anaerobic Jars Set Up Using the Anaero-Pack
• Anaerobic Glove Box
• Anaerobe Culture Triage
• Anaerobe Culture Triage (cont.)
• Identification
• Colonial Morphology
• Pigmented Colonies
• Rapid Identification Using Gram Stain
• Identification
• 16S Ribosomal RNA Sequence of Bacteroides fragilis
• Antimicrobial Susceptibility Testing
• AST Methods
• Illustration of the Components of the E-test Method
• Susceptibility Testing by E-test
• Anaerobe Antimicrobial Panels
• References
• Special Thanks
• Questions